Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| tumor necrosis factor |
Polyclonal |
IgG |
Rabbit |
Mouse |
ELISA, Neu, IP, IHC-P, WB |
Immunogen affinity purified. |
Immunogen
|
| E. coli-derived mouse TNF alpha recombinant protein(Position: L80-L235). |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
TNF |
Protein
|
tumor necrosis factor |
Uniprot ID
|
P06804 |
Function
|
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. |
Tissue Specificity
|
|
Sub-cellular localization
|
Cell membrane; Single-pass type II membrane protein. |
Sequence Similarities
|
|
Aliases
|
APC1 antibody|APC1 protein antibody|Cachectin antibody|DIF antibody|Differentiation inducing factor antibody|Macrophage cytotoxic factor antibody|MCF antibody|Necrosin antibody|TNF a antibody|TNF alpha antibody|TNF antibody|TNF Macrophage Derived antibody|TNF Monocyte Derived antibody|TNF Superfamily Member 2 antibody|TNF superfamily, member 2 antibody|TNF, macrophage derived antibody|TNF, monocyte derived antibody|TNF-a antibody| TNF-alpha antibody|TNFA antibody|TNFA_HUMAN antibody|TNFSF2 antibody|Tumor necrosis factor alpha antibody|Tumor necrosis factor antibody|Tumor necrosis factor ligand superfamily member 2 antibody|Tumor Necrosis Factor Precursor antibody|Tumor Necrosis Factor, Membrane Form antibody|Tumor necrosis factor, soluble form antibody|Tumour Necrosis Factor Alpha antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Mouse |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
0.5-1μg/ml |
Mouse |
AssaySolutio’s IHC/ICC Detection kit |
| ELISA |
0.1-0.5μg/ml |
Mouse |
Sandwich ELISA format |
| Neutralization |
Mouse |
Mouse |
|
| Immunoprecipitation |
Mouse |
Mouse |
|
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
