Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| cholinergic receptor, muscarinic 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human muscarinic Acetylcholine Receptor 1(303-317aa KMPMVDPEAQAPTKQ), identical to the related mouse sequence, and different from the related rat sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CHRM1 |
Protein |
Muscarinic acetylcholine receptor M1 |
Uniprot ID |
P11229 |
Function |
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. |
Tissue Specificity |
|
Sub-cellular localization |
Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. |
Sequence Similarities |
|
Aliases |
Cholinergic receptor muscarinic 1 antibody|CHRM 1 antibody|CHRM1 antibody|HM 1 antibody|HM1 antibody|M1 antibody|M1 muscarinic cholinergic receptor antibody|MGC30125 antibody|Muscarinic acetylcholine receptor M1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-muscarinic Acetylcholine Receptor 1 antibody, ASA-B1331, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Lane 3: U87 Cell Lysate
Lane 4: SHG Cell Lysate
Lane 5: NEURO Cell Lysate
Lane 6: HELA Cell Lys
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Lane 3: U87 Cell Lysate
Lane 4: SHG Cell Lysate
Lane 5: NEURO Cell Lysate
Lane 6: HELA Cell Lys
Anti-muscarinic Acetylcholine Receptor 1 antibody, ASA-B1331, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
