Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| myosin, heavy chain 7, cardiac muscle, beta |
Monoclonal |
IgG |
Mouse |
Human, Mouse, Rabbit, Rat |
IHC-P, WB |
Ascites |
Immunogen
|
| Human skeletal muscle myosin purified from myofibrils. |
Properties
Form
|
Liquid |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
|
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
MYH7 |
Protein
|
Myosin-7 |
Uniprot ID
|
Human(P12883), Mouse(Q91Z83), Rat(P02564) |
Function
|
Muscle contraction. |
Tissue Specificity
|
Both wild type and variant Gln-403 are detected in skeletal muscle (at protein level). |
Sub-cellular localization
|
Cytoplasm, myofibril. Note: Thick filaments of the myofibrils. |
Sequence Similarities
|
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family. |
Aliases
|
CMH1|MPD1|SPMD|SPMM|CMD1S|MYHCB|Myosin heavy chain 7|Myosin heavy chain slow isoform|MyHC-slow|Myosin heavy chain, cardiac muscle beta isoform|MyHC-beta |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.5-2μg/ml |
Human, mouse, rabbit, rat |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
1-2μg/ml |
Human, mouse, rabbit, rat |
AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Myosin(Skeletal, Slow) antibody (monoclonal), ASA-B1343, Western blotting
WB: Rat Skeletal Muscle Tissue Lysate
-antibody-(monoclonal)-ASA-B1343-1.jpg)
-antibody-(monoclonal)-ASA-B1343-2.jpg)
