N-Cadherin Antibody (monoclonal)

N-Cadherin Antibody (monoclonal)

$330.00

In stock

SKU: ASA-B1345 Category:

Description

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
cadherin 2, type 1, N-cadherin(neuronal) Monoclonal IgG Mouse Chicken, Human, Mouse, Rabbit, Rat IHC-F, WB Ascites

Immunogen
Affinity purified chicken heart A-CAM.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.

Concentration

 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 CDH2

Protein

 Cadherin-2

Uniprot ID

 Human(P19022), Mouse(P15116), Rat(Q9Z1Y3)

Function

 Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity).

Tissue Specificity

Sub-cellular localization

 Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Aliases

 Cadherin2|CDHN|NCAD|CD325|CDw325|Neural cadherin

Application Details

Application Concentration* Species Validated Using**
Western blot 2μg/ml Human, mouse, rat, rabbit, chicken AssaySolutio’s ECL kit
Immunohistochemistry(Frozen Section) 4μg/ml Human, mouse, rat, rabbit, chicken AssaySolutio’s IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information

Anti-N-Cadherin antibody (monoclonal), ASA-B1345, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Medulla Oblongata Tissue Lysate

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