Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| solute carrier family 12(sodium/potassium/chloride transporters), member 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human NKCC1(223-241aa RIDHYRHTAAQLGEKLLRP), identical to the related mouse and rat sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
SLC12A2 |
Protein |
Solute carrier family 12 member 2 |
Uniprot ID |
P55011 |
Function |
Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. |
Tissue Specificity |
Expressed in many tissues. |
Sub-cellular localization |
Membrane; Multi-pass membrane protein. |
Sequence Similarities |
|
Aliases |
Basolateral Na-K-Cl symporter antibody|BSC antibody|BSC2 antibody|Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1 antibody|mBSC2 antibody|MGC104233 antibody|NKCC1 antibody|S12A2_HUMAN antibody|SLC12A2 antibody|sodium-potassium-chloride cotransporter 1 antibody|solute carrier family 12(sodium/potassium/chloride transporters) member 2 antibody|Solute carrier family 12 member 2 antibody|sy-ns antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NKCC1 antibody, ASA-B1381, Western blotting
WB: HELA Cell Lysate
WB: HELA Cell Lysate
Anti-NKCC1 antibody, ASA-B1381, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Anti-NKCC1 antibody, ASA-B1381, IHC(P)
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IHC(P): Rat Intestine Tissue
