Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| glutamate receptor, ionotropic, N-methyl D-aspartate 2A | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human NMDAR2A(1360-1376aa, DHTSDNPFLHSHRDDQR), different from the related mouse sequence by three amino acids, and from the related rat sequences by four amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
GRIN2A |
Protein |
Glutamate[NMDA] receptor subunit epsilon-1 |
Uniprot ID |
Q12879 |
Function |
NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits. |
Tissue Specificity |
|
Sub-cellular localization |
Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. |
Sequence Similarities |
|
Aliases |
Glutamate[NMDA] receptor subunit epsilon 1 antibody|Glutamate[NMDA] receptor subunit epsilon-1 antibody|Glutamate receptor antibody|Glutamate receptor ionotropic N methyl D aspartate 2A antibody|GRIN 2A antibody|GRIN2A antibody|HNR2A antibody|N methyl D aspartate receptor channel, subunit epsilon 1 antibody|N Methyl D Aspartate Receptor Subtype 2A antibody|N methyl D aspartate receptor subunit 2A antibody|N-methyl D-aspartate receptor subtype 2A antibody|NMDA receptor subtype 2A antibody|NMDA Receptor Type 2A antibody|NMDAR 2A antibody|NMDAR2A antibody|NMDE1_HUMAN antibody|NR 2A antibody|NR2A antibody|OTTHUMP00000160135 antibody|OTTHUMP00000174531 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NMDAR2A antibody, ASA-B1387, Western blotting
All lanes: Anti NMDAR2A (ASA-B1387) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 165KD
Observed bind size: 165KD
All lanes: Anti NMDAR2A (ASA-B1387) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 165KD
Observed bind size: 165KD
Anti-NMDAR2A antibody, ASA-B1387, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
