Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| NADPH oxidase 1 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human NOX1(354-374aa, HIRAAGDWTENLIRAFEQQYS). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
NOX1 |
Protein |
NADPH oxidase 1(NOX-1) |
Uniprot ID |
Q9Y5S8 |
Function |
NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. |
Tissue Specificity |
NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH- 1S is detected only in colon and colon carcinoma cells. |
Sub-cellular localization |
Cell projection, invadopodium membrane. |
Sequence Similarities |
Contains 1 FAD-binding FR-type domain. |
Aliases |
GP91 2 antibody|Mitogenic oxidase(pyridine nucleotide dependent superoxide generating) antibody|Mitogenic oxidase 1 antibody|MOX-1 antibody|MOX1 antibody|NADH/NADPH mitogenic oxidase subunit P65 MOX antibody|NADH/NADPH mitogenic oxidase subunit P65-MOX antibody|NADPH oxidase 1 antibody|NADPH oxidase 1 variant NOH 1L antibody|NADPH oxidase homolog 1 antibody|NOH 1 antibody|NOH-1 antibody|NOH1 antibody|NOX-1 antibody|Nox1 antibody|NOX1_HUMAN antibody|RP1 146H21.1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NOX1 antibody, ASA-B1401, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
Anti-NOX1 antibody, ASA-B1401, Western blotting
All lanes: Anti NOX1 (ASA-B1401) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 65KD
Observed bind size: 65KD
All lanes: Anti NOX1 (ASA-B1401) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 65KD
Observed bind size: 65KD
