Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| cytochrome b-245, beta polypeptide | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human NOX2(442-459aa YWLCRDTHAFEWFADLLQ), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CYBB |
Protein |
Cytochrome b-245 heavy chain |
Uniprot ID |
P04839 |
Function |
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. |
Tissue Specificity |
Detected in neutrophils (at protein level). |
Sub-cellular localization |
Cell membrane; Multi-pass membrane protein. |
Sequence Similarities |
Contains 1 FAD-binding FR-type domain. |
Aliases |
AMCBX2 antibody|CGD antibody|CGD91-phox antibody|CY24B_HUMAN antibody|CYBB antibody|Cytochrome b 245, beta polypeptide antibody|Cytochrome b(558) beta chain antibody|Cytochrome b(558) subunit beta antibody|Cytochrome b-245 heavy chain antibody|Cytochrome b558 subunit beta antibody|GP91 PHOX antibody|gp91-1 antibody|gp91-phox antibody|GP91PHOX antibody|Heme-binding membrane glycoprotein gp91phox antibody|NADPH oxidase 2 antibody|Neutrophil cytochrome b 91 kDa polypeptide antibody|NOX2 antibody|p22 phagocyte B-cytochrome antibody|P91 PHOX antibody|p91-PHOX antibody|Superoxide-generating NADPH oxidase heavy chain subunit antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NOX2/gp91phox antibody, ASA-B1402, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: JURKAT Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: SMMC Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: JURKAT Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: SMMC Cell Lysate
Anti-NOX2/gp91phox antibody, ASA-B1402, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
