Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| nuclear factor, erythroid 2-like 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human Nrf2 recombinant protein (Position: Q14-D178). Human Nrf2 shares 79% and 82% amino acid (aa) sequence identity with mouse and rat Nrf2, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
NFE2L2 |
Protein |
Nuclear factor erythroid 2-related factor 2 |
Uniprot ID |
Q16236 |
Function |
Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. |
Tissue Specificity |
Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle. |
Sub-cellular localization |
Cytoplasm, cytosol. Nucleus. Note: Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents. |
Sequence Similarities |
Belongs to the bZIP family. CNC subfamily. |
Aliases |
erythroid derived 2 antibody|HEBP1 antibody|NF E2 related factor 2 antibody|NF-E2-related factor 2 antibody|NF2L2_HUMAN antibody|NFE2 related factor 2 antibody|NFE2-related factor 2 antibody|NFE2L2 antibody|Nrf 2 antibody|NRF2 antibody|Nuclear factor antibody|nuclear factor erythroid 2 like 2 antibody|Nuclear factor erythroid 2 related factor 2 antibody|Nuclear factor erythroid 2-related factor 2 antibody|Nuclear factor erythroid derived 2 like 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Nrf2 antibody, ASA-B1414, Western blotting
All lanes: Anti Nrf2 (ASA-B1414) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 4: Mouse Lung Tissue Lysate at 50ug
Predicted bind size: 68KD
Observed bind size: 100KD
All lanes: Anti Nrf2 (ASA-B1414) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 4: Mouse Lung Tissue Lysate at 50ug
Predicted bind size: 68KD
Observed bind size: 100KD
Anti- Nrf2 antibody, ASA-B1414, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
