Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| bone gamma-carboxyglutamate (gla) protein | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human Osteocalcin recombinant protein (Position: Y52-V100). Human Osteocalcin shares 69% and 86% amino acid (aa) sequences identity with mouse and rat Osteocalcin, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
BGLAP |
Protein |
Osteocalcin |
Uniprot ID |
P02818 |
Function |
Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. |
Tissue Specificity |
|
Sub-cellular localization |
Secreted. |
Sequence Similarities |
Belongs to the osteocalcin/matrix Gla protein family. |
Aliases |
BGLAP antibody|BGP antibody|Bone gamma carboxyglutamate gla protein osteocalcin antibody|Bone gamma carboxyglutamate protein antibody|Bone Gla protein antibody|Gamma carboxyglutamic acid containing protein antibody|Gamma-carboxyglutamic acid-containing protein antibody|OC antibody|OSTCN_HUMAN antibody|Osteocalcin antibody|OTTHUMP00000016586 antibody|PMF1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Osteocalcin antibody, ASA-B1437–1.jpg
IHC(P): Human Osteosarcoma Tissue
IHC(P): Human Osteosarcoma Tissue
Anti-Osteocalcin antibody, ASA-B1437–2.jpg
All lanes: Anti-Osteocalcin(ASA-B1437) at 0.5ug/ml
WB: Recombinant Human Osteocalcin Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti-Osteocalcin(ASA-B1437) at 0.5ug/ml
WB: Recombinant Human Osteocalcin Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
