Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| cyclin-dependent kinase inhibitor 2A |
Monoclonal |
IgG |
Mouse |
Human |
IHC-F, ICC, WB |
Ascites |
Immunogen
|
| Recombinant human p16 protein. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
CDKN2A |
Protein
|
Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3 |
Uniprot ID
|
Human(P42771), Mouse(P51480), Rat(Q9R0Z3) |
Function
|
Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein. |
Tissue Specificity
|
Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific. |
Sub-cellular localization
|
Cytoplasm. |
Sequence Similarities
|
Belongs to the CDKN2 cyclin-dependent kinase inhibitor family. |
Aliases
|
ARF|MLM|P14|P16|P19|CMM2|INK4|MTS1|TP16|CDK4I|CDKN2|INK4A|MTS-1|P14ARF|P19ARF|P16INK4|P16INK4A|P16-INK4A|CDK4 INHIBITOR|cyclin-dependent kinase inhibitor 2A(melanoma, p16, inhibits CDK4)|Cyclin-dependent kinase 4 inhibitor A|Multiple tumor suppressor 1|Collaborator of ARF |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.5-1μg/ml |
Human |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Frozen Section) |
1-2μg/ml |
Human |
AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry |
Suitable |
Human |
AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
