Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| p21 protein(Cdc42/Rac)-activated kinase 6 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human PAK6(16-32aa QNFQHRVHTSFDPKEGK), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
PAK6 |
Protein |
Serine/threonine-protein kinase PAK 6 |
Uniprot ID |
Q9NQU5 |
Function |
Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Inhibits also ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD. |
Tissue Specificity |
Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast. |
Sub-cellular localization |
Cytoplasm. Nucleus. Note: Cotranslocates into nucleus with AR in response to androgen induction. |
Sequence Similarities |
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. |
Aliases |
CDKN1A activated kinase 6 antibody|EC 2.7.1.37 antibody|p21 activated protein kinase 6 antibody|p21 protein(Cdc42/Rac)-activated kinase 6 antibody|p21(CDKN1A) activated kinase 6 antibody|p21-activated kinase 6 antibody|p21activated kinase 6 antibody|PAK 5 antibody|PAK 6 antibody|PAK-5 antibody|PAK-6 antibody|PAK5 antibody|Pak6 antibody|PAK6_HUMAN antibody|Serine threonine protein kinase PAK 6 antibody|Serine/threonine protein kinase PAK 6 antibody|Serine/threonine protein kinase PAK6 antibody|Serine/threonine-protein kinase PAK 6 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PAK6 antibody, ASA-B1471, Western blotting
All lanes: Anti PAK6 (ASA-B1471) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: 293T Whole Cell Lysate at 40ug
Lane 3: RAJI Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 75KD
Observed bind size: 75KD
All lanes: Anti PAK6 (ASA-B1471) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: 293T Whole Cell Lysate at 40ug
Lane 3: RAJI Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 75KD
Observed bind size: 75KD
Anti-PAK6 antibody, ASA-B1471, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
