Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| phosphoglycerate kinase 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human PGK1(166-180aa FGTAHRAHSSMVGVN), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
PGK1 |
Protein |
Phosphoglycerate kinase 1 |
Uniprot ID |
P00558 |
Function |
In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
Belongs to the phosphoglycerate kinase family. |
Aliases |
Cell migration-inducing gene 10 protein antibody|MGC117307 antibody|MGC8947 antibody|MIG10 antibody|pgk1 antibody|PGK1_HUMAN antibody|PGKA antibody|Phosphoglycerate kinase 1 antibody|Primer recognition protein 2 antibody|PRP 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Rat, Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PGK1 antibody, ASA-B1515, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Lung Tissue Lysate
Lane 4: A431 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: A549 Cell Lysate
Lane 8: JURKAT Cell Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Lung Tissue Lysate
Lane 4: A431 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: A549 Cell Lysate
Lane 8: JURKAT Cell Lysate
Anti-PGK1 antibody, ASA-B1515, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
