Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| ATP-binding cassette, sub-family B(MDR/TAP), member 1 |
Monoclonal |
IgG |
Mouse |
Human |
IHC-P, IHC-F, ICC, WB |
Ascites |
Immunogen
|
| Mixture of human and hamster drug-resistant whole cells and crude plasma membranes. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
1853 |
Protein
|
Multidrug resistance protein 1 |
Uniprot ID
|
Human(P08183), Mouse(P21447), Rat(P43245) |
Function
|
Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. |
Tissue Specificity
|
Expressed in liver, kidney, small intestine and brain. |
Sub-cellular localization
|
Cell membrane ; Multi-pass membrane protein. |
Sequence Similarities
|
Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. |
Aliases
|
CLCS|MDR1|P-GP|PGY1|ABC20|CD243|GP170|ATP-binding cassette sub-family B member 1;P-glycoprotein 1 |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.5-1μg/ml |
Human |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
1-2μg/ml |
Human |
AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) |
1-2μg/ml |
Human |
AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry |
Suitable |
Human |
AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
