Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| protein phosphatase 2, catalytic subunit, alpha isozyme | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human PP2A-alpha recombinant protein (Position: M1-L309). Human PP2A-alpha shares 100% amino acid (aa) sequence identity with both mouse and rat PP2A-alpha. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
PPP2CA |
Protein |
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform |
Uniprot ID |
P67775 |
Function |
PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at ‘Ser-259’. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
|
Aliases |
PP2A alpha antibody|PP2A-alpha antibody|PP2AA_HUMAN antibody|PP2Aalpha antibody|PP2Ac antibody|PP2CA antibody|PP2Calpha antibody|PPP2CA antibody|Protein phosphatase 2 (formerly 2A) catalytic subunit alpha isoform antibody|Protein phosphatase 2 catalytic subunit alpha isoform antibody|Protein phosphatase 2, catalytic subunit, alpha isozyme antibody|Protein phosphatase 2A catalytic subunit, alpha isoform antibody|Replication protein C antibody|RP C antibody|RP-C antibody|RPC antibody|Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody|Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- PP2A-alpha antibody, ASA-B1556, Western blotting
All lanes: Anti PP2A-alpha (ASA-B1556) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Mouse Kidney Tissue Lysate at 50ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: Rat Liver Tissue Lysate at 50ug
Lane 5: NIH Whole Cell Lysate at 40ug
Lane 6: PC-12 Whole Cell Lysate at 40ug
Lane 7: 293T Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
All lanes: Anti PP2A-alpha (ASA-B1556) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Mouse Kidney Tissue Lysate at 50ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: Rat Liver Tissue Lysate at 50ug
Lane 5: NIH Whole Cell Lysate at 40ug
Lane 6: PC-12 Whole Cell Lysate at 40ug
Lane 7: 293T Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
Anti- PP2A-alpha antibody, ASA-B1556, IHC(P)
IHC(P): Mouse Brain Tissue
IHC(P): Mouse Brain Tissue
Anti- PP2A-alpha antibody, ASA-B1556, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
