Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| KIT ligand | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human SCF(26-44aa DEGICRNRVTNNVKDVTKLV). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
KITLG |
Protein |
Kit ligand |
Uniprot ID |
P21583 |
Function |
Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. |
Tissue Specificity |
|
Sub-cellular localization |
Isoform 1: Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities |
Belongs to the SCF family. |
Aliases |
C kit ligand antibody|C-kit ligand antibody|Ckit ligand antibody|DKFZp686F2250 antibody|familial progressive hyperpigmentation 2 antibody|FPH2 antibody|KIT ligand antibody|Kitl antibody|KITLG antibody|KL 1 antibody|KL1 antibody|Mast cell growth factor antibody|MGF antibody|SCF antibody|SF antibody|SHEP7 antibody|sKITLG antibody|Soluble KIT ligand antibody|Steel factor antibody|Stem cell factor antibody|Stem cell factor precursor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SCF antibody, ASA-B1651, Western blotting
All lanes: Anti SCF (ASA-B1651) at 0.5ug/ml
Lane 1: Recombinant Human SCF Protein 10ng
Lane 2: Recombinant Human SCF Protein 5ng
Lane 3: Recombinant Human SCF Protein 2.5ng
Predicted bind size: 18.4KD
Observed bind size: 18.4KD
All lanes: Anti SCF (ASA-B1651) at 0.5ug/ml
Lane 1: Recombinant Human SCF Protein 10ng
Lane 2: Recombinant Human SCF Protein 5ng
Lane 3: Recombinant Human SCF Protein 2.5ng
Predicted bind size: 18.4KD
Observed bind size: 18.4KD
Anti-SCF antibody, ASA-B1651, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
