Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| succinate dehydrogenase complex, subunit C, integral membrane protein, 15kDa | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human SDHC(35-50aa KEEMERFWNKNIGSNR), different from the related mouse and rat sequences by three amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
SDHC |
Protein |
Succinate dehydrogenase cytochrome b560 subunit, mitochondrial |
Uniprot ID |
Q99643 |
Function |
Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). |
Tissue Specificity |
|
Sub-cellular localization |
Mitochondrion inner membrane; Multi-pass membrane protein. |
Sequence Similarities |
Belongs to the cytochrome b560 family. |
Aliases |
CYB560 antibody|C560_HUMAN antibody|CYBL antibody|Cytochrome B large subunit of complex II antibody|Integral membrane protein CII-3 antibody|mitochondrial antibody|PGL3 antibody|QPs-1 antibody|QPs1 antibody|SDH3 antibody|sdhC antibody|Succinate dehydrogenase complex subunit C antibody|Succinate dehydrogenase complex subunit C integral membrane protein 15kDa antibody|Succinate dehydrogenase cytochrome b560 subunit antibody|Succinate dehydrogenase cytochrome b560 subunit mitochondrial antibody|Succinate dehydrogenase cytochrome b560 subunit, mitochondrial precursor antibody|Succinate-ubiquinone oxidoreductase cytochrome B large subunit antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SDHC antibody, ASA-B1660, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: HELA Tissue Lysate
Lane 3: JURKAT Cell Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: HELA Tissue Lysate
Lane 3: JURKAT Cell Lysate
Anti-SDHC antibody, ASA-B1660, IHC(P)
IHC(P): Rat Gaster Tissue
IHC(P): Rat Gaster Tissue
Anti-SDHC antibody, ASA-B1660, IHC(P)
IHC(P): Human Gastric Cancer Tissue
IHC(P): Human Gastric Cancer Tissue
