Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| calcyclin binding protein | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human SIAH Interacting Protein(160-175aa NTRWDYLTQVEKECKE), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CACYBP |
Protein |
Calcyclin-binding protein(CacyBP/hCacyBP) |
Uniprot ID |
Q9HB71 |
Function |
May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1). |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Contains 1 CS domain. |
Aliases |
CACYBP antibody|Calcyclin binding protein antibody|GIG 5 antibody|GIG5 antibody|Growth inhibiting gene 5 antibody|Growth inhibiting gene 5 protein antibody|hCacyBP antibody|MGC87971 antibody|PNAS 107 antibody|PNAS107 antibody|RP1 102G20.6 antibody|S100A6 binding protein antibody|S100A6BP antibody|SIP antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SIAH Interacting Protein antibody, ASA-B1690, Western blotting
All lanes: Anti SIAH Interacting Protein (ASA-B1690) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: SW620 Whole Cell Lysate at 40ug
Lane 7: 293T Whole Cell Lysate at 40ug
Predicted bind size: 26KD
Observed bind size:
All lanes: Anti SIAH Interacting Protein (ASA-B1690) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: SW620 Whole Cell Lysate at 40ug
Lane 7: 293T Whole Cell Lysate at 40ug
Predicted bind size: 26KD
Observed bind size:
Anti-SIAH Interacting Protein antibody, ASA-B1690, ICC
ICC: A549 Cell
ICC: A549 Cell
Anti-SIAH Interacting Protein antibody, ASA-B1690,IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
