Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| solute carrier family 16 (monocarboxylate transporter), member 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminal of human SLC16A3(438-465aa VDLREVEHFLKAEPEKNGEVVHTPETSV), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
SLC1A1 |
Protein |
Monocarboxylate transporter 4 |
Uniprot ID |
O15427 |
Function |
Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate (By similarity). |
Tissue Specificity |
Highly expressed in skeletal muscle. |
Sub-cellular localization |
Cell membrane; Multi-pass membrane protein. |
Sequence Similarities |
Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. |
Aliases |
MCT 4 antibody|MCT3 antibody|MCT4 antibody|Monocarboxylate transporter 3 antibody|Monocarboxylate transporter 4 antibody|MOT4_HUMAN antibody|SLC16A3 antibody|Solute carrier family 16 member 3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SLC16A3 antibody, ASA-B1706, Western blotting
All lanes: Anti SLC16A3 (ASA-B1706) at 0.5ug/ml
WB: Recombinant Human SLC16A3 Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
All lanes: Anti SLC16A3 (ASA-B1706) at 0.5ug/ml
WB: Recombinant Human SLC16A3 Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
Anti- SLC16A3 antibody, ASA-B1706, Western blotting
All lanes: Anti SLC16A3 (ASA-B1706) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: K562 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: HL-60 Whole Cell Lysate at 40ug
Lane 6: Rat Brain Tissue Lysate at 50ug
Lane 7: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 49KD
Observed bind size: 49KD
All lanes: Anti SLC16A3 (ASA-B1706) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: K562 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: HL-60 Whole Cell Lysate at 40ug
Lane 6: Rat Brain Tissue Lysate at 50ug
Lane 7: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 49KD
Observed bind size: 49KD
Anti- SLC16A3 antibody, ASA-B1706, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
