Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| suppressor of cytokine signaling 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human SOCS1(197-211aa NPVLRDYLSSFPFQI), identical to the related mouse and rat sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
SOCS1 |
Protein |
Suppressor of cytokine signaling 1(SOCS-1) |
Uniprot ID |
O15524 |
Function |
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein- tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SOCS1 appears to be a negative regulator in IGF1R signaling pathway. |
Tissue Specificity |
Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes. |
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Contains 1 SH2 domain. |
Aliases |
CISH 1 antibody|CISH1 antibody|Cytokine Inducible SH2 Protein 1 antibody|JAB antibody|JAK Binding Protein antibody|JAK-binding protein antibody|Janus kinase binding protein antibody|SOCS 1 antibody|SOCS-1 antibody|Socs1 antibody|SOCS1_HUMAN antibody|SSI 1 antibody|SSI-1 antibody|SSI1 antibody|STAT Induced STAT Inhibitor 1 antibody|STAT-induced STAT inhibitor 1 antibody|Suppressor of cytokine signaling 1 antibody|Supressor of cytokine Signalling 1 antibody|TEC Interacting Protein 3 antibody|Tec-interacting protein 3 antibody|TIP 3 antibody|TIP-3 antibody|TIP3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SOCS1 antibody, ASA-B1744, Western blotting
Lane 1: HT1080 Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 1: HT1080 Cell Lysate
Lane 2: COLO320 Cell Lysate
Anti-SOCS1 antibody, ASA-B1744, IHC(P)
IHC(P): Rat Spleen Tissue
IHC(P): Rat Spleen Tissue
