Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| superoxide dismutase 2, mitochondrial | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human SOD2(45-62aa QIMQLHHSKHHAAYVNNL), identical to the related mouse sequence and different from the related rat sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
SOD2 |
Protein |
Superoxide dismutase[Mn], mitochondrial |
Uniprot ID |
P04179 |
Function |
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. |
Tissue Specificity |
|
Sub-cellular localization |
Mitochondrion matrix. |
Sequence Similarities |
Belongs to the iron/manganese superoxide dismutase family. |
Aliases |
Indophenoloxidase B antibody|IPO B antibody|IPOB antibody|Manganese containing superoxide dismutase antibody|Manganese SOD antibody|Manganese superoxide dismutase antibody|Mangano superoxide dismutase antibody|Mn SOD antibody|Mn superoxide dismutase antibody|MNSOD antibody|MVCD6 antibody|SOD 2 antibody|SOD-2 antibody|SOD2 antibody|SODM_HUMAN antibody|Superoxide dismutase[Mn] mitochondrial antibody|Superoxide dismutase[Mn] mitochondrial precursor antibody|Superoxide dismutase[Mn], mitochondrial antibody|Superoxide dismutase 2 mitochondrial antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SOD2 antibody, ASA-B1751, Western blotting
All lanes: Anti SOD2 (ASA-B1751) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Intestine Tissue Lysate at 50ug
Lane 3: Rat Lung Tissue Lysate at 50ug
Lane 4: Rat Heart Tissue Lysate at 50ug
Lane 5: SMMC Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: SW620 Whole Cell Lysate at 40ug
Lane 9: A549 Whole Cell Lysate at 40ug
Pr
All lanes: Anti SOD2 (ASA-B1751) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Intestine Tissue Lysate at 50ug
Lane 3: Rat Lung Tissue Lysate at 50ug
Lane 4: Rat Heart Tissue Lysate at 50ug
Lane 5: SMMC Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: SW620 Whole Cell Lysate at 40ug
Lane 9: A549 Whole Cell Lysate at 40ug
Pr
Anti-SOD2 antibody, ASA-B1751, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
Anti-SOD2 antibody, ASA-B1751, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
