Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| tropomyosin 1(alpha) |
Monoclonal |
IgG |
Mouse |
Chicken, Human, Rat |
IHC-P, IHC-F, WB |
Ascites |
Immunogen
|
| Chicken muscle tropomyosin. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
TPM1 |
Protein
|
Tropomyosin alpha-1 chain |
Uniprot ID
|
Human(P09493), Mouse(P58771), Rat(P04692) |
Function
|
Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. |
Tissue Specificity
|
Detected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle. |
Sub-cellular localization
|
Cytoplasm, cytoskeleton. |
Sequence Similarities
|
|
Aliases
|
CMH3|TMSA|CMD1Y|C15orf13|HTM-alpha|Alpha-tropomyosin|Tropomyosin-1 |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
1-2μg/ml |
Human, rat, chicken. |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
2-4μg/ml |
Human, rat, chicken. |
AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) |
2-4μg/ml |
Human, rat, chicken. |
AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
