Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| thyroid stimulating hormone receptor | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human TSH Receptor(30-47aa ECHQEEDFRVTCKDIQRI). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
TSHR |
Protein |
Thyrotropin receptor |
Uniprot ID |
P16473 |
Function |
Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5). |
Tissue Specificity |
Expressed in the thyroid. |
Sub-cellular localization |
Cell membrane; Multi-pass membrane protein. |
Sequence Similarities |
Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily. |
Aliases |
CHNG1 antibody|hTSHR I antibody|hTSHRI antibody|LGR 3 antibody|LGR3 antibody|MGC75129 antibody|Thyroid adenoma hyperfunctioning antibody|Thyroid carcinoma with thyrotoxicosis antibody|Thyroid Stimulating Hormone Receptor antibody|Thyrotropin Receptor antibody|Thyrotropin receptor I antibody|TSH R antibody|TSHR antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TSH Receptor antibody, ASA-B1941, IHC(P)
IHC(P): Human Thyroid Cancer Tissue
IHC(P): Human Thyroid Cancer Tissue
Anti-TSH Receptor antibody, ASA-B1941, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human TSHR, 33.5KD(162aa tag+ M1-T136)
Lane 1: Recombinant Human TSHR Protein 10ng
Lane 2: Recombinant Human TSHR Protein 5ng
Lane 3: Recombinant Human TSHR Protein 2.5ng
Recombinant Protein Detection Source: E.coli derived -recombinant Human TSHR, 33.5KD(162aa tag+ M1-T136)
Lane 1: Recombinant Human TSHR Protein 10ng
Lane 2: Recombinant Human TSHR Protein 5ng
Lane 3: Recombinant Human TSHR Protein 2.5ng
