Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| vascular cell adhesion molecule 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
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| A synthetic peptide corresponding to a sequence at the C-terminus of mouse VCAM1(653-671aa YTIRQAQLQDAGIYECESK), different from the related human sequence by three amino acids, and from the related rat sequence by two amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
VCAM1 |
Protein |
Vascular cell adhesion protein 1 |
Uniprot ID |
P29533 |
Function |
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. |
Tissue Specificity |
Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Expressed in the bone marrow. |
Sub-cellular localization |
Isoform 1: Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities |
Contains 7 Ig-like C2-type (immunoglobulin-like) domains. |
Aliases |
CD 106 antibody|CD106 antibody|CD106 Antigen antibody|DKFZp779G2333 antibody|INCAM 100 antibody|INCAM-100 antibody|L1CAM antibody|MGC99561 antibody|V-CAM 1 antibody|Vascular Cell Adhesion Molecule 1 antibody|Vascular cell adhesion protein 1 antibody|VCAM 1 antibody|VCAM-1 antibody|VCAM1 antibody|VCAM1_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-VCAM1 antibody, ASA-B1955, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue
Anti-VCAM1 antibody, ASA-B1955, Western blotting
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
