Vimentin Antibody (monoclonal)

Vimentin Antibody (monoclonal)

$330.00

In stock

SKU: ASA-B1974 Category:

Description

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
vimentin Monoclonal IgG Mouse Human, Pig, Rabbit, Rat IHC-P, WB Ascites

Immunogen
Pig eye lens vimentin.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.

Concentration

 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 VIM

Protein

 Vimentin

Uniprot ID

 Human(P08670), Mouse(P20152), Rat(P31000)

Function

 Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Tissue Specificity

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt’s lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines.

Sub-cellular localization

 Cytoplasm.

Sequence Similarities

 Belongs to the intermediate filament family.

Aliases

Application Details

Application Concentration* Species Validated Using**
Western blot 0.5-1μg/ml Human, rabbit, pig, rat. AssaySolutio’s ECL kit
Immunohistochemistry(Paraffin-embedded Section) 1.5-2.5μg/ml Human, rabbit, pig, rat. AssaySolutio’s IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information

Anti-Vimentin antibody (monoclonal), ASA-B1974, IHC(P)
IHC(P): Human Mammary Cancer Tissue

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