Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| WNT1 inducible signaling pathway protein 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human WISP1(354-367aa ADLESYPDFSEIAN), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
WISP1 |
Protein |
WNT1-inducible-signaling pathway protein 1 |
Uniprot ID |
O95388 |
Function |
Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. |
Tissue Specificity |
Expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Isoform 2 is expressed predominantly in scirrhous gastric carcinoma and, weakly in placenta. Overexpression is associated with several cancers including breast cancer and colon tumors. Isoform 2 is overexpressed in scirrhous gastric carcinoma. |
Sub-cellular localization |
Secreted. |
Sequence Similarities |
Belongs to the CCN family. |
Aliases |
CCN 4 antibody|CCN family member 4 antibody|CCN4 antibody|WISP 1 antibody|WISP 1c antibody|WISP 1i antibody|WISP 1tc antibody|WISP1c antibody|WISP1i antibody|WISP1tc antibody|WNT 1 induced secreted protein 1 antibody|Wnt 1 induced secreted protein antibody|WNT 1 inducible signaling pathway protein 1 antibody|Wnt 1 signaling pathway protein 1 antibody|Wnt-1 inducible signaling pathway protein 1 antibody|WNT1 induced secreted protein 1 antibody|Wnt1 induced secreted protein antibody|WNT1 inducible signaling pathway protein 1 antibody|Wnt1 signaling pathway protein 1 antibody|WNT1-inducible-signaling pathway protein 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-WISP1 antibody, ASA-B1986, IHC(P)
IHC(P): Rat Kidney Tissue
IHC(P): Rat Kidney Tissue
Anti-WISP1 antibody, ASA-B1986, IHC(P)
IHC(P): Rat Pancreas Tissue
IHC(P): Rat Pancreas Tissue
Anti-WISP1 antibody, ASA-B1986, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
