Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| cyclin D2 |
Polyclonal |
IgG |
Rabbit |
Human |
IHC-P |
Immunogen affinity purified. |
Immunogen
|
| E.coli-derived human Cyclin D2 recombinant protein (Position: H157-L289). Human Cyclin D2 shares 87% and 89% amino acid (aa) sequences identity with mouse and rat Cyclin D2, respectively. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
CCND2 |
Protein
|
G1/S-specific cyclin-D2 |
Uniprot ID
|
P30279 |
Function
|
Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity). |
Tissue Specificity
|
|
Sub-cellular localization
|
Nucleus. |
Sequence Similarities
|
Belongs to the cyclin family. Cyclin D subfamily. |
Aliases
|
CCND 2 antibody|CCND2 antibody|CCND2_HUMAN antibody|CyclinD2 antibody|G1/S specific cyclin D2 antibody|G1/S-specific cyclin-D2 antibody|KIAK0002 antibody|MGC102758 antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Immunohistochemistry(Paraffin-embedded Section) |
0.5-1μg/ml |
Human |
AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Cyclin D2 antibody, ASA-B0536, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
