Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| TIMP metallopeptidase inhibitor 2 |
Polyclonal |
IgG |
Rabbit |
Human, Mouse, Rat |
ELISA, IHC-P |
Immunogen affinity purified. |
Immunogen
|
| A synthetic peptide corresponding to a sequence at the C-terminus of human TIMP2(205-220aa RGAAPPKQEFLDIEDP), identical to the related mouse and rat sequences. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
TIMP2 |
Protein
|
Metalloproteinase inhibitor 2 |
Uniprot ID
|
P16035 |
Function
|
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. |
Tissue Specificity
|
|
Sub-cellular localization
|
Secreted. |
Sequence Similarities
|
Belongs to the protease inhibitor I35 (TIMP) family. |
Aliases
|
CSC 21K antibody|CSC-21K antibody|CSC21K antibody|Metalloproteinase inhibitor 2 antibody|Metalloproteinase inhibitor 2 precursor antibody|TIMP 2 antibody|TIMP metallopeptidase inhibitor 2 antibody|TIMP-2 antibody|TIMP2 antibody|TIMP2_HUMAN antibody|Tissue Inhibitor of Metalloproteinase 2 antibody|Tissue inhibitor of metalloproteinases 2 antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Immunohistochemistry(Paraffin-embedded Section) |
0.5-1μg/ml |
Human Mouse, Rat |
AssaySolutio’s IHC/ICC Detection kit |
| ELISA |
0.1-0.5μg/ml |
Human Mouse, Rat |
Sandwich ELISA format |
AssaySolution recommends Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TIMP2 antibody, ASA-B1863, IHC(P)
IHC(P): Human Mammary Cancer Tissue
