Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| ADAM metallopeptidase with thrombospondin type 1 motif, 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
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| A synthetic peptide corresponding to a sequence at the C-terminus of human ADAMTS4(820-837aa RRAQILEILRRRPWAGRK), different from the related rat and mouse sequences by three amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
ADAMTS4 |
Protein |
A disintegrin and metalloproteinase with thrombospondin motifs 4(ADAM-TS 4/ADAM-TS4/ADAMTS-4) |
Uniprot ID |
O75173 |
Function |
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the ‘392-Glu- |
Tissue Specificity |
Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles. Isoform 2 is detected in osteoarthritic synovium. |
Sub-cellular localization |
Secreted, extracellular space, extracellular matrix . |
Sequence Similarities |
Contains 1 disintegrin domain. |
Aliases |
A disintegrin and metalloproteinase with thrombospondin motifs 4 antibody|A disintegrin like and metalloprotease(reprolysin type) with thrombospondin type 1 motif 4 antibody|A disintegrin like and metalloprotease with thrombospondin type 1 motif 4 antibody|ADAM metallopeptidase with thrombospondin type 1 motif 4 antibody|ADAM TS 4 antibody|ADAM TS4 antibody|ADAM-TS 4 antibody|ADAM-TS4 antibody|ADAMTS 2 antibody|ADAMTS 4 antibody|ADAMTS-4 antibody|ADAMTS2 antibody|ADAMTS4 antibody|ADAMTS4 protein antibody|ADMP 1 antibody|ADMP-1 antibody|ADMP1 antibody|Aggrecanase 1 antibody|Aggrecanase-1 antibody|Aggrecanase1 antibody|ATS4_HUMAN antibody|KIAA0688 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ADAMTS4 antibody, ASA-B0038, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
