Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| zinc finger E-box binding homeobox 1 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human AREB6(108-123aa KDDECESDAENEQNHD), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
ZEB1 |
Protein |
Zinc finger E-box-binding homeobox 1 |
Uniprot ID |
P37275 |
Function |
Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5′-CANNTG-3′). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. |
Tissue Specificity |
Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. |
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family. |
Aliases |
AREB 6 antibody|BZP antibody|Delta crystallin enhancer binding factor 1 antibody|DELTA EF1 antibody|FECD6 antibody|MGC133261 antibody|Negative regulator of IL 2 antibody|Negative regulator of IL2 antibody|NIL 2 A antibody|NIL 2 A zinc finger protein antibody|NIL 2A antibody|NIL-2-A zinc finger protein antibody|NIL2A antibody|Posterior polymorphous corneal dystrophy 3 antibody|PPCD3 antibody|Represses interleukin 2 expression antibody|TCF 8 antibody|TCF-8 antibody|TCF8 antibody|Transcription factor 8(represses interleukin 2 expression) antibody|Transcription factor 8 antibody|ZEB 1 antibody|ZEB antibody|ZEB1 antibody|ZEB1_HUMAN antibody|ZFHEP antibody|ZFHX 1A antibody|ZFHX1A antibody|Zinc finger E box binding homeobox 1 antibody|Zinc finger E-box-binding homeobox 1 antibody|Zinc finger homeodomain enhancer binding protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-AREB6 antibody, ASA-B0149, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: SMMC Cell Lysate
Lane 4: A549 Cell Lysate
Lane 1: MCF-7 Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: SMMC Cell Lysate
Lane 4: A549 Cell Lysate
