Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| actin, beta | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human beta Actin(2-19aaDDDIAALVVDNGSGMCKA), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
ACTB |
Protein |
Actin, cytoplasmic 1 |
Uniprot ID |
P60709 |
Function |
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm, cytoskeleton. |
Sequence Similarities |
Belongs to the actin family. |
Aliases |
A26C1A antibody|A26C1B antibody|ACTB antibody|ACTB_HUMAN antibody|Actin cytoplasmic 1 antibody|Actx antibody|b-actin antibody|beta cytoskeletal actin antibody|Beta-actin antibody|E430023M04Rik antibody|MGC128179 antibody|PS1TP5 binding protein 1 antibody|PS1TP5BP1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-beta Actin antibody, ASA-B0218, Western blotting
All lanes: Anti beta Actin (ASA-B0218) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Ovary Tissue Lysate at 50ug
Lane 3: Rat Heart Tissue Lysate at 50ug
Lane 4: Rat Brain Tissue Lysate at 50ug
Lane 5: A453 Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: MCF-7 Whole Cell Lysate at 40ug
Lane 8: SMMC Whole Cell Lysate at 40ug
Lane 9: SW620 Whole Cell Lysate at 40
All lanes: Anti beta Actin (ASA-B0218) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Ovary Tissue Lysate at 50ug
Lane 3: Rat Heart Tissue Lysate at 50ug
Lane 4: Rat Brain Tissue Lysate at 50ug
Lane 5: A453 Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: MCF-7 Whole Cell Lysate at 40ug
Lane 8: SMMC Whole Cell Lysate at 40ug
Lane 9: SW620 Whole Cell Lysate at 40
