Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| endoglin | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human CD105 recombinant protein (Position: E26-I384). Human CD105 shares 67% amino acid (aa) sequence identity with mouse CD105. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
ENG |
Protein |
Endoglin |
Uniprot ID |
P17813 |
Function |
Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF- beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3. |
Tissue Specificity |
Endoglin is restricted to endothelial cells in all tissues except bone marrow. |
Sub-cellular localization |
Membrane; Single-pass type I membrane protein. |
Sequence Similarities |
|
Aliases |
AI528660 antibody|AI662476 antibody|CD 105 antibody|CD105 antibody|CD105 antigen antibody|EGLN_HUMAN antibody|END antibody|Endoglin antibody|ENG antibody|FLJ41744 antibody|HHT1 antibody|ORW antibody|ORW1 antibody|Osler Rendu Weber syndrome 1 antibody|RP11 228B15.2 antibody|S-endoglin antibody|SN6 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD105 antibody, ASA-B0342–1.jpg
All lanes: Anti-CD105(ASA-B0342) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 71KD
Observed bind size: 71KD
All lanes: Anti-CD105(ASA-B0342) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 71KD
Observed bind size: 71KD
