Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| CD14 molecule | Polyclonal | IgG | Rabbit | Mouse | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of mouse CD14(152-165aa TRDAWLAELQQWLK). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CD14 |
Protein |
Monocyte differentiation antigen CD14 |
Uniprot ID |
P10810 |
Function |
In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules (By similarity). |
Tissue Specificity |
|
Sub-cellular localization |
Cell membrane; Lipid-anchor, GPI-anchor. |
Sequence Similarities |
|
Aliases |
CD 14 antibody|CD_antigen=CD14 antibody|CD14 antibody|CD14 antibody|CD14 antigen antibody|CD14 molecule antibody|CD14_HUMAN antibody|LPS-R antibody|Mo2 antibody|Monocyte differentiation antigen CD14 antibody|Monocyte differentiation antigen CD14 urinary form antibody|Monocyte differentiation antigen CD14, membrane-bound form antibody|Myeloid cell specific leucine rich glycoprotein antibody|Myeloid cell-specific leucine-rich glycoprotein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD14 antibody, ASA-B0350, Western blotting
Lane 1: Mouse Thymus Tissue Lysate
Lane 2: Mouse Spleen Tissue Lysate
Lane 1: Mouse Thymus Tissue Lysate
Lane 2: Mouse Spleen Tissue Lysate
