Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| cell division cycle 37 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Cdc37(361-376aa DPLLEAVPKTGDEKDV), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CDC37 |
Protein |
Hsp90 co-chaperone Cdc37 |
Uniprot ID |
Q16543 |
Function |
Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
Belongs to the CDC37 family. |
Aliases |
CDC 37 antibody|CDC37 antibody|CDC37 cell division cycle 37 homolog antibody|CDC37 cell division cycle 37 S cerevisiae homolog antibody|Cdc37 homolog antibody|CDC37 protein antibody|CDC37_HUMAN antibody|CDC37A antibody|Cell division cycle 37 homolog antibody|Hsp90 chaperone protein kinase targeting subunit antibody|Hsp90 chaperone protein kinase targeting subunit p50Cdc37 antibody|Hsp90 chaperone protein kinase-targeting subunit antibody|Hsp90 co chaperone Cdc37 antibody|Hsp90 co-chaperone Cdc37 antibody|p50 antibody|p50Cdc37 antibody|S cerevisiae hypothetical protein CDC37 antibody|S. cerevisiae hypothetical protein CDC37 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Cdc37 antibody, ASA-B0420, Western blotting
All lanes: Anti Cdc37(ASA-B0420) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: 293T Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 44KD
All lanes: Anti Cdc37(ASA-B0420) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: 293T Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 44KD
