Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| v-myc myelocytomatosis viral oncogene homolog(avian) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human c-Myc(421-439aa RKRREQLKHKLEQLRNSCA), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
MYC |
Protein |
Myc proto-oncogene protein |
Uniprot ID |
P01106 |
Function |
Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5′- CAC[GA]TG-3′. Activates the transcription of growth-related genes. |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus, nucleoplasm. |
Sequence Similarities |
Contains 1 bHLH (basic helix-loop-helix) domain. |
Aliases |
AU016757 antibody|Avian myelocytomatosis viral oncogene homolog antibody|bHLHe39 antibody|c Myc antibody|c Myc Epitope Tag antibody|c Myc Tag antibody|Class E basic helix-loop-helix protein 39 antibody|MRTL antibody|Myc antibody|Myc Epitope Tag antibody|Myc protein antibody|Myc proto oncogene protein antibody|Myc proto-oncogene protein antibody|MYC_HUMAN antibody|Myc2 antibody|Myelocytomatosis oncogene antibody|Niard antibody|Nird antibody|Oncogene Myc antibody|OTTHUMP00000158589 antibody|Proto-oncogene c-Myc antibody|RNCMYC antibody|Transcription factor p64 antibody|Transcriptional regulator Myc-A antibody|V-Myc avian myelocytomatosis viral oncogene homolog antibody|v-myc myelocytomatosis viral oncogene homolog(avian) antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-c-Myc antibody, ASA-B0252, Western blotting
All lanes: Anti c-Myc(ASA-B0252) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 49KD
Observed bind size: 49KD
All lanes: Anti c-Myc(ASA-B0252) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 49KD
Observed bind size: 49KD
