Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| collagen, type IV, alpha 1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Collagen IV(245-263aa QAQVQEKGDFATKGEKGQK), different from the related mouse and rat sequences by three amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
COL4A1 |
Protein |
Collagen alpha-1(IV) chain |
Uniprot ID |
P02462 |
Function |
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a ‘chicken-wire’ meshwork together with laminins, proteoglycans and entactin/nidogen. |
Tissue Specificity |
Highly expressed in placenta. |
Sub-cellular localization |
Secreted, extracellular space, extracellular matrix, basement membrane. |
Sequence Similarities |
Belongs to the type IV collagen family. |
Aliases |
Arresten antibody|Canstatin antibody|CO4A1_HUMAN antibody|COL4A1 antibody|COL4A1 NC1 domain antibody|Collagen Alpha 1(IV) Chain antibody|collagen alpha-1(IV) chain antibody|Collagen IV Alpha 1 Polypeptide antibody|Collagen Of Basement Membrane Alpha 1 Chain antibody|Collagen Type IV Alpha 1 antibody|DKFZp686I14213 antibody|FLJ22259 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- COL4A1 antibody, ASA-B0474, Western blotting
All lanes: Anti COL4A1 (ASA-B0474) at 0.5ug/ml
Lane 1: MM231 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: SW620Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Predicted bind size: 160KD
Observed bind size: 160KD
All lanes: Anti COL4A1 (ASA-B0474) at 0.5ug/ml
Lane 1: MM231 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: SW620Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Predicted bind size: 160KD
Observed bind size: 160KD
