Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| cyclin A1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human Cyclin A1 recombinant protein (Position: D64-E263). Human Cyclin A1 shares 78.5% and 79.5% amino acid (aa) sequence identity with mouse and rat Cyclin A1, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CCNA1 |
Protein |
Cyclin-A1 |
Uniprot ID |
P78396 |
Function |
May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. |
Tissue Specificity |
Very high levels in testis and very low levels in brain. Also found in myeloid leukemia cell lines. |
Sub-cellular localization |
Nucleus . |
Sequence Similarities |
Belongs to the cyclin family. Cyclin AB subfamily. |
Aliases |
CCN A1 antibody|CCNA 1 antibody|CCNA1 antibody|CCNA1_HUMAN antibody| Cyclin-A1 antibody| CyclinA1 antibody|G2/mitotic specific cyclin A1 antibody| MGC132235 antibody|MGC159139 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Cyclin A1 antibody, ASA-B0527, Western blotting
All lanes: Anti Cyclin A1 (ASA-B0527) at 0.5ug/ml
WB: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 52KD
Observed bind size: 52KD
All lanes: Anti Cyclin A1 (ASA-B0527) at 0.5ug/ml
WB: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 52KD
Observed bind size: 52KD
