Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| tumor necrosis factor receptor superfamily, member 10a | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human DR4(110-125aa TIKLHDQSIGTQQWEH). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
TNFRSF10A |
Protein |
Tumor necrosis factor receptor superfamily member 10A |
Uniprot ID |
O00220 |
Function |
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF- kappa-B. |
Tissue Specificity |
Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K-562 erythroleukemia cells, MCF-7 breast carcinoma cells and activated T-cells. |
Sub-cellular localization |
Membrane; Single-pass type I membrane protein. |
Sequence Similarities |
Contains 1 death domain. |
Aliases |
Apo 2 antibody|Apo2 antibody|CD 261 antibody|CD261 antibody|CD261 antigen antibody|Cytotoxic ligand TRAIL receptor antibody|Cytotoxic TRAIL receptor antibody|Death receptor 4 antibody|DR 4 antibody|DR4 antibody|MGC9365 antibody|NF related apoptosis-inducing ligand receptor 1 antibody|T10A antibody|TNF related apoptosis inducing ligand receptor 1 antibody|TNF-related apoptosis-inducing ligand receptor 1 antibody|TNFRSF10A antibody|TR10A_HUMAN antibody|TRAIL R1 antibody|TRAIL receptor 1 antibody|TRAIL-R1 antibody|TRAILR 1 antibody|TRAILR1 antibody|Tumor necrosis factor receptor superfamily member 10a antibody|Tumor necrosis factor receptor superfamily member 10A precursor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-DR4 antibody, ASA-B0609, Western blotting
Lane 1: SW620 Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: HT1080 Cell Lysate
Lane 1: SW620 Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: HT1080 Cell Lysate
