Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| dopamine receptor D1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human DRD1 recombinant protein (Position: K249-T446). Human DRD1 shares 91% amino acid (aa) sequence identity with both mouse and rat DRD1. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
DRD1 |
Protein |
D(1A) dopamine receptor |
Uniprot ID |
P21728 |
Function |
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. |
Tissue Specificity |
Detected in caudate, nucleus accumbens and in the olfactory tubercle. |
Sub-cellular localization |
Cell membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Note: Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14. |
Sequence Similarities |
|
Aliases |
D(1A) dopamine receptor antibody|D1A dopamine receptor antibody|DADR antibody|DADR antibody|Dopamine D1 receptor antibody|Dopamine Receptor D1 antibody|DR D1 antibody|DR D1A antibody|DRD 1 antibody|DRD 1A antibody|DRD1 antibody|DRD1_HUMAN antibody|DRD1A antibody|DRD1A antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- DRD1 antibody, ASA-B0611, Western blotting
All lanes: Anti DRD1 (ASA-B0611) at 0.5ug/ml
WB: Recombinant Human DRD1 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti DRD1 (ASA-B0611) at 0.5ug/ml
WB: Recombinant Human DRD1 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
Anti- DRD1 antibody, ASA-B0611, Western blotting
All lanes: Anti DRD1 (ASA-B0611) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: Hela Whole Cell Lysate at 40ug
Lane 5: SHG Whole Cell Lysate at 40ug
Predicted bind size: 49KD
Observed bind size: 49KD
All lanes: Anti DRD1 (ASA-B0611) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: Hela Whole Cell Lysate at 40ug
Lane 5: SHG Whole Cell Lysate at 40ug
Predicted bind size: 49KD
Observed bind size: 49KD
