Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| early growth response 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human EGR2(153-168aa TMSQTQPDLDHLYSPP), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
EGR2 |
Protein |
E3 SUMO-protein ligase EGR2 |
Uniprot ID |
P11161 |
Function |
Sequence-specific DNA-binding transcription factor. Binds to two specific DNA sites located in the promoter region of HOXA4. |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Belongs to the EGR C2H2-type zinc-finger protein family. |
Aliases |
AT591 antibody|CMT1D antibody|CMT4E antibody|DKFZp686J1957 antibody|E3 SUMO-protein ligase EGR2 antibody|Early growth response 2 antibody|Early growth response protein 2 antibody|EGR-2 antibody|egr2 antibody|EGR2_HUMAN antibody|FLJ14547 antibody|KROX 20 Drosophila homolog antibody|Krox 20 homolog Drosophila antibody|KROX-20, Drosophila, homolog(early growth response-2) antibody|KROX20 antibody|Krox20 protein antibody|Zinc finger protein Krox-20 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-EGR2 antibody, ASA-B0638, Western blotting
Lane 1: NIH3T3 Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 1: NIH3T3 Cell Lysate
Lane 2: MCF-7 Cell Lysate
