Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| chemokine(C-C motif) ligand 11 | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse Eotaxin(73-93aa CADPKKKWVQDATKHLDQKLQ), identical to the related rat sequence. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CCL11 |
Protein |
Eotaxin |
Uniprot ID |
P48298 |
Function |
In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils. |
Tissue Specificity |
Expressed constitutively in the thymus. Expression inducible in the lung (type I alveolar epithelial cells), intestine, heart, spleen, kidney. |
Sub-cellular localization |
Secreted. |
Sequence Similarities |
|
Aliases |
C-C motif chemokine 11 antibody|CCL 11 antibody|CCL11 antibody|CCL11_HUMAN antibody|chemokine(C-C motif) ligand 11 antibody|Chemokine C C motif ligand 11 antibody|Chemokine CC Motif Ligand 11 antibody|Chemokine ligand 11 antibody|Eosinophil Chemotactic Protein antibody|Eotaxin antibody|eotaxin-1 antibody|MGC22554 antibody|SCYA 11 antibody|SCYA11 antibody|Small inducible cytokine A11 antibody|Small inducible cytokine subfamily A(Cys Cys) member 11 antibody|small inducible cytokine subfamily A(Cys-Cys), member 11(eotaxin) antibody|Small inducible cytokine subfamily A member 11 antibody|Small-inducible cytokine A11 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Eotaxin antibody, ASA-B0650, Western blotting
Lane 1: Recombinant mouse Eotaxin Protein 10ng
Lane 2: Recombinant mouse Eotaxin Protein 5ng
Lane 3: Recombinant mouse Eotaxin Protein 2.5ng
Lane 4: Recombinant mouse Eotaxin Protein 1.25ng
Lane 1: Recombinant mouse Eotaxin Protein 10ng
Lane 2: Recombinant mouse Eotaxin Protein 5ng
Lane 3: Recombinant mouse Eotaxin Protein 2.5ng
Lane 4: Recombinant mouse Eotaxin Protein 1.25ng
