Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| alpha-2-HS-glycoprotein | Polyclonal | IgG | Rabbit | Mouse | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived mouse Fetuin A recombinant protein (Position: A19-I345). Mouse Fetuin A shares 57% and 85% amino acid (aa) sequence identity with human and rat Fetuin A, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
2017 |
Protein |
Alpha-2-HS-glycoprotein |
Uniprot ID |
P29699 |
Function |
Probably involved in differentiation. |
Tissue Specificity |
Liver is the major site of synthesis, but fetuin is also expressed in limb buds and other extrahepatic tissues during development. |
Sub-cellular localization |
Secreted. |
Sequence Similarities |
Belongs to the fetuin family. |
Aliases |
59 kDa bone sialic acid containing protein antibody|A2HS antibody|Aa2 066 antibody|AHS antibody|Ahsg antibody|Alpha 2 HS glycoprotein antibody|Alpha 2 HS glycoprotein chain A antibody|Alpha 2 HS glycoprotein chain B antibody|Alpha 2 Z globulin antibody|Alpha 2HS glycoprotein antibody|Alpha-2-HS-glycoprotein chain B antibody|Alpha-2-Z-globulin antibody|alpha2 Heremans Schmid glycoprotein antibody|Asialofetuin antibody|Ba alpha 2 glycoprotein antibody|Ba-alpha-2-glycoprotein antibody|BSP antibody|Countertrypin antibody|FETUA antibody|FETUA_HUMAN antibody|Fetuin A antibody|Fetuin, mouse, homolog of antibody|Fetuin-A antibody|FetuinA antibody|Glycoprotein PP63 antibody|HSGA antibody|pp63 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Fetuin A antibody, ASA-B0697, Western blotting
All lanes: Anti FetuinA (ASA-B0697) at 0.5ug/ml
WB: Recombinant Mouse FetuinA Protein 0.5ng
Predicted bind size: 37KD
Observed bind size: 37KD
All lanes: Anti FetuinA (ASA-B0697) at 0.5ug/ml
WB: Recombinant Mouse FetuinA Protein 0.5ng
Predicted bind size: 37KD
Observed bind size: 37KD
Anti- Fetuin A antibody, ASA-B0697, Western blotting
All lanes: Anti FetuinA (ASA-B0697) at 0.5ug/ml
Lane 1: NIH3T3 Whole Cell Lysate at 40ug
Lane 2: NEURO Whole Cell Lysate at 40ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti FetuinA (ASA-B0697) at 0.5ug/ml
Lane 1: NIH3T3 Whole Cell Lysate at 40ug
Lane 2: NEURO Whole Cell Lysate at 40ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 39KD
Observed bind size: 39KD
