Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| glucose-6-phosphate dehydrogenase | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human G6PD recombinant protein (Position: E315-L515). Human G6PD shares 95% and 96% amino acid (aa) sequences identity with mouse and rat G6PD, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
G6PD |
Protein |
Glucose-6-phosphate 1-dehydrogenase |
Uniprot ID |
P11413 |
Function |
Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. |
Tissue Specificity |
Isoform Long is found in lymphoblasts, granulocytes and sperm. |
Sub-cellular localization |
|
Sequence Similarities |
Belongs to the glucose-6-phosphate dehydrogenase family. |
Aliases |
G6PD antibody|G6PD_HUMAN antibody|G6PD1 antibody|G6pdx antibody|Glucose 6 phosphate 1 dehydrogenase antibody|Glucose 6 phosphate dehydrogenase antibody|Glucose 6 phosphate dehydrogenase, G6PD antibody|Glucose-6-phosphate 1-dehydrogenase antibody|MET19 antibody|POS10 antibody|Zwf1p antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- G6PD antibody, ASA-B0745, Western blotting
All lanes: Anti G6PD (ASA-B0745) at 0.5ug/ml
WB: Recombinant Human G6PD Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti G6PD (ASA-B0745) at 0.5ug/ml
WB: Recombinant Human G6PD Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti- G6PD antibody, ASA-B0745, Western blotting
All lanes: Anti G6PD (ASA-B0745) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: SKOV Whole Cell Lysate at 40ug
Lane 4: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 59KD
Observed bind size: 59KD
All lanes: Anti G6PD (ASA-B0745) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: SKOV Whole Cell Lysate at 40ug
Lane 4: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 59KD
Observed bind size: 59KD
