Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| glutamate decarboxylase 1 (brain, 67kDa) | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human GAD67 recombinant protein (Position: N14-D122). Human GAD67 shares 95% amino acid (aa) sequence identity with both mouse and rat GAD67. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
GAD1 |
Protein |
Glutamate decarboxylase 1 |
Uniprot ID |
Q99259 |
Function |
Catalyzes the production of GABA. |
Tissue Specificity |
Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. |
Sub-cellular localization |
|
Sequence Similarities |
Belongs to the group II decarboxylase family. |
Aliases |
67 kDa glutamic acid decarboxylase antibody|DCE1 antibody|DCE1_HUMAN antibody|EC 4.1.1.15 antibody|FLJ45882 antibody|GAD 1 antibody|GAD 67 antibody|GAD antibody|GAD-67 antibody|GAD1 antibody|Glutamate decarboxylase 1 (brain 67kD) antibody|Glutamate decarboxylase 1 antibody|Glutamate decarboxylase 1 brain 67kD antibody|Glutamate decarboxylase 1 brain 67kDa antibody|Glutamate decarboxylase 67 kDa isoform antibody|OTTHUMP00000041055 antibody|SCP antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- GAD67 antibody, ASA-B0751, Western blotting
All lanes: Anti GAD67 (ASA-B0751) at 0.5ug/ml
WB: Recombinant Human GAD67 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti GAD67 (ASA-B0751) at 0.5ug/ml
WB: Recombinant Human GAD67 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti- GAD67 antibody, ASA-B0751, Western blotting
All lanes: Anti GAD67 (ASA-B0751) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Hela Whole Cell Lysate at 40ug
Lane 3: Colo320 Whole Cell Lysate at 40ug
Predicted bind size: 67KD
Observed bind size: 67KD
All lanes: Anti GAD67 (ASA-B0751) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Hela Whole Cell Lysate at 40ug
Lane 3: Colo320 Whole Cell Lysate at 40ug
Predicted bind size: 67KD
Observed bind size: 67KD
