Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| golgi-associated, gamma adaptin ear containing, ARF binding protein 1; golgi-associated, gamma adaptin ear containing, ARF binding protein 2; golgi-associated, gamma adaptin ear containing, ARF binding protein 3 |
Monoclonal |
IgG |
Mouse |
Human, Mouse, Rat |
ICC, WB |
Ascites |
Immunogen
|
| Synthetic gamma-tubulin peptide(amino acids 38-53), conjugated to KLH. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
GGA1; GGA2; GGA3 |
Protein
|
ADP-ribosylation factor-binding protein GGA1; ADP-ribosylation factor-binding protein GGA2; ADP-ribosylation factor-binding protein GGA3 |
Uniprot ID
|
Human(Q9UJY5), Mouse(Q8R0H9), Rat(Q5FVF3), Human(Q9UJY4), Mouse(Q6P5E6), Rat(Q4G038), Human(Q9NZ52), Mouse(Q8BMI3), Rat(D3ZHG8) |
Function
|
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF- dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. |
Tissue Specificity
|
Ubiquitously expressed. |
Sub-cellular localization
|
Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. |
Sequence Similarities
|
Belongs to the GGA protein family. |
Aliases
|
VEAR|KIAA1080|KIAA0154|Gamma-adaptin-related protein 1|Gamma-adaptin-related protein 2|Golgi-localized, gamma ear-containing, ARF-binding protein 2|VHS domain and ear domain of gamma-adaptin |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.25-0.5μg/ml |
Human, mouse, rat |
AssaySolutio’s ECL kit |
| Immunocytochemistry |
Suitable |
Human, mouse, rat |
AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for ICC. *Blocking peptide can be purchased at $65. Contact us for more information
