Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| GATA binding protein 5 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human GATA5 recombinant protein (Position: M1-A397). Human GATA5 shares 75% amino acid (aa) sequence identity with mouse GATA5. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
GATA5 |
Protein |
Transcription factor GATA-5 |
Uniprot ID |
Q9BWX5 |
Function |
Transcription factor required during cardiovascular development (PubMed:23289003). Plays an important role in the transcriptional program(s) that underlies smooth muscle cell diversity (By similarity). Binds to the functionally important CEF-1 nuclear protein binding site in the cardiac-specific slow/cardiac troponin C transcriptional enhancer (PubMed:25543888). |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus . |
Sequence Similarities |
Contains 2 GATA-type zinc fingers. |
Aliases |
GATA binding protein 5 antibody|GATA-binding factor 5 antibody|GATA5 antibody|GATA5_HUMAN antibody|Transcription factor GATA-5 antibody|bB379O24.1 antibody|GATAS antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- GATA5 antibody, ASA-B0769, Western blotting
All lanes: Anti GATA5 (ASA-B0769) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Rat Ovary Tissue Lysate at 50ug
Predicted bind size: 42KD
Observed bind size: 42KD
All lanes: Anti GATA5 (ASA-B0769) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Rat Ovary Tissue Lysate at 50ug
Predicted bind size: 42KD
Observed bind size: 42KD
