Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| colony stimulating factor 3 (granulocyte) | Polyclonal | IgG | Rabbit | Human | ELISA, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E. coli-derived human G-CSF recombinant protein (Position: S38-P207). Human G-CSF shares 76% amino acid (aa) sequence identity with mouse G-CSF. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CSF3 |
Protein |
Granulocyte colony-stimulating factor |
Uniprot ID |
P09919 |
Function |
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. |
Tissue Specificity |
|
Sub-cellular localization |
Secreted. |
Sequence Similarities |
Belongs to the IL-6 superfamily. |
Aliases |
C17orf33 antibody|Colony stimulating factor 3 (granulocyte) antibody|CSF 3 antibody|CSF beta antibody|CSF3 antibody|CSF3_HUMAN antibody|CSF3OS antibody|Csfg antibody|Filgrastim antibody|G-CSF antibody|GCSA antibody|GCSF antibody|Granulocyte colony stimulating factor antibody|Granulocyte colony-stimulating factor antibody|Lenograstim antibody|Macrophage granulocyte inducer 2 antibody|MGC45931 antibody|MGI 2 antibody|Pluripoietin antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-G-CSF antibody, ASA-B0742, Western blotting
All lanes: Anti G-CSF (ASA-B0742) at 0.5ug/ml
WB: K562 Whole Cell Lysate at 40ug
Predicted bind size: 22KD
Observed bind size: 22KD
All lanes: Anti G-CSF (ASA-B0742) at 0.5ug/ml
WB: K562 Whole Cell Lysate at 40ug
Predicted bind size: 22KD
Observed bind size: 22KD
