Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| gap junction protein, beta 2, 26kDa |
Polyclonal |
IgG |
Rabbit |
Human, Mouse, Rat |
WB |
Immunogen affinity purified. |
Immunogen
|
| A synthetic peptide corresponding to a sequence at the N-terminus of human GJB2(1-16aa, MDWGTLQTILGGVNKH), different from the related mouse and rat sequences by two amino acids. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
GJB2 |
Protein
|
Gap junction beta-2 protein |
Uniprot ID
|
P29033 |
Function
|
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. |
Tissue Specificity
|
|
Sub-cellular localization
|
Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. |
Sequence Similarities
|
Belongs to the connexin family. Beta-type (group I) subfamily. |
Aliases
|
connexin 26 antibody|Connexin-26 antibody|cx26 antibody|CXB2_HUMAN antibody|DFNA3 antibody|DFNB1 antibody|Gap junction beta-2 protein antibody|GJB2 antibody|HID antibody|KID antibody|NSRD1 antibody|PPK antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Human, Rat Mouse |
AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-GJB2 antibody, ASA-B0787, Western blotting
WB: Rat Liver Tissue Lysate
