Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| antigen identified by monoclonal antibody Ki-67 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Ki67(3234-3256aa KKAEDNVCVKKIRTRSHRDSEDI). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
MKI67 |
Protein |
Antigen KI-67 |
Uniprot ID |
P46013 |
Function |
Thought to be required for maintaining cell proliferation. |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Contains 1 FHA domain. |
Aliases |
Antigen identified by monoclonal antibody Ki 67 antibody|Antigen KI-67 antibody|Antigen KI67 antibody|KI67_HUMAN antibody|KIA antibody|MKI67 antibody|Proliferation related Ki 67 antigen antibody|RP11-380J17.2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Ki67 antibody, ASA-B1118, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human Ki67 50.3KD(162aa tag+ K2967-I3256)
Lane 1: Recombinant Human Ki67 Protein 10ng
Lane 2: Recombinant Human Ki67 Protein 5ng
Lane 3: Recombinant Human Ki67 Protein 2.5ng
Recombinant Protein Detection Source: E.coli derived -recombinant Human Ki67 50.3KD(162aa tag+ K2967-I3256)
Lane 1: Recombinant Human Ki67 Protein 10ng
Lane 2: Recombinant Human Ki67 Protein 5ng
Lane 3: Recombinant Human Ki67 Protein 2.5ng
