Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human KRAS(164-178aa/b RKHKEKMSKDGKKKK), identical to the related mouse and rat sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
KRAS |
Protein |
GTPase Kras |
Uniprot ID |
P01116 |
Function |
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838). |
Tissue Specificity |
|
Sub-cellular localization |
Cell membrane. |
Sequence Similarities |
Belongs to the small GTPase superfamily. Ras family. |
Aliases |
c Kirsten ras protein antibody|c-K-ras antibody|c-Ki-ras antibody|Cellular c Ki ras2 proto oncogene antibody|cK Ras antibody|GTPase KRas antibody|K RAS p21 protein antibody|K RAS2A antibody|K RAS2B antibody|K RAS4A antibody|K RAS4B antibody|K-Ras 2 antibody|KI RAS antibody|Ki-Ras antibody|KIRSTEN MURINE SARCOMA VIRUS 2 antibody|Kirsten rat sarcoma 2 viral(v Ki ras2) oncogene homolog antibody|KRAS antibody|KRAS1 antibody|KRAS2 antibody|N-terminally processed antibody|NS antibody|NS3 antibody|Oncogene KRAS2 antibody|p21ras antibody|PR310 cK ras oncogene antibody|RASK_HUMAN antibody|RASK2 antibody|Transforming protein p21 antibody|v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-KRAS antibody, ASA-B1133, Western blotting
Lane 1: HT1080 Cell Lysate
Lane 2: SW620 Cell Lysate
Lane 1: HT1080 Cell Lysate
Lane 2: SW620 Cell Lysate
